14 C Incorporation into the Fatty Acids and Aliphatic Hydrocarbons of Sarcina lutea

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14-C incorporation into the fatty acids and aliphatic hydrocarbons of Sarcina lutea.

An initial investigation into the mechanism of hydrocarbon biosynthesis in Sarcina lutea was performed by measuring the amounts of (14)C incorporated into the hydrocarbons and fatty acids by use of a combination gas chromatograph and high-temperature gas-flow ionization apparatus. Uniformly labeled l-isoleucine-(14)C was predominantly incorporated into the anteiso-branched chains. Palmitate-16-...

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Incorporation of Photosensitive Fatty Acids into Phospholipids

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Incorporation of radioactive citrate into fatty acids.

BRADY AND GURIN 1 and DITURI AND GURIN 2 were the first to show that soluble enzymes of pigeon liver could synthesize long-chain fatty acids from acetate and that citrate stimulated this process. These results were confirmed and extended to a number of other tissues by other workers and purification of these systems has led to a rather detailed understanding of fatty acid synthesisa, 4. One obs...

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Effects of aliphatic fatty acids

octanoate or decanoate per mol of albumin caused a decrease in dye binding (dye/protein molar ratio 1 : 1), which, in contrast with additional fatty acid, was very pronounced: 1-8mol of palmitate or stearate resulted in a small, and apparently linear, displacement of Phenol Red. The displacement effect of 1-5 mol of oleate, linoleate or linolenate per mol of albumin was comparable with that of ...

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The incorporation of citrate carbon into fatty acids.

Long-chain fatty acids are synthesized from citrate in an undialyzed soluble enzyme system from pigeon liver at a rate comparable to that observed when acetate is used as a precursor. The first enzymic step for citrate incorporation is probably its breakdown to acetyl-CoA and oxaloacetate catalyzed by the citrate~cleavage enzyme. Experiments with variously labeled citrate show good incorporatio...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1967

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.94.2.349-358.1967